Difference between revisions of "Part:BBa K900000:Design"
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===Source=== | ===Source=== | ||
| − | Synthesized in house and cloned into addgene plasmid [http://www.addgene.org/11911/ l11911] Previously engineered version of RFP from Subach et al. | + | Synthesized in house and cloned into addgene plasmid [http://www.addgene.org/11911/ l11911] Previously engineered version of RFP from Subach et al., 2009. |
===References=== | ===References=== | ||
Subach, F. V. et al. Photoactivatable mCherry for high-resolution two-color fluorescence microscopy. Nature Methods 6, 153–159 (2009). | Subach, F. V. et al. Photoactivatable mCherry for high-resolution two-color fluorescence microscopy. Nature Methods 6, 153–159 (2009). | ||
Revision as of 22:31, 3 October 2012
Design Notes
The gene was codon optimized for yeast and internal restriction sites were removed for to compatibility with our Golden Gate cloning scheme.
Source
Synthesized in house and cloned into addgene plasmid [http://www.addgene.org/11911/ l11911] Previously engineered version of RFP from Subach et al., 2009.
References
Subach, F. V. et al. Photoactivatable mCherry for high-resolution two-color fluorescence microscopy. Nature Methods 6, 153–159 (2009).