Difference between revisions of "Part:BBa K892011"

Latest revision as of 21:47, 3 October 2012

sfGFP-osmY

osmY fused to superfolder GFP with a serine-glycine linker, with sfGFP upstream of osmY. This part was made by swapping out the mamI coding sequence from BBa_K590016 and replacing it with BBa_K892008 using Gibson assembly. This part was characterized using the method outlined in the [http://2012.igem.org/Team:Washington/Protocols/osmY_Assay Washington 2012 wiki], the data summary is shown below.

Each histogram depicts the average normalized fluorescence values for cells plus supernatant, cells only, and supernatant only. The image in the center is a visual representation of the presented data.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 94

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K892011 short</partinfo>
-Osmy fused to superfolder GFP with a serine-glycine linker, with SfGFP upstream of Osmy
+osmY fused to superfolder GFP with a serine-glycine linker, with sfGFP upstream of osmY. This part was made by swapping out the mamI coding sequence from [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590016 BBa_K590016] and replacing it with [https://parts.igem.org/wiki/index.php?title=Part:BBa_K892008 BBa_K892008] using Gibson assembly. This part was characterized using the method outlined in the [http://2012.igem.org/Team:Washington/Protocols/osmY_Assay Washington 2012 wiki], the data summary is shown below.
+[[Image:Washington_osmY_Assay_Results.png|border|950px|center|thumb|Each histogram depicts the average normalized fluorescence values for cells plus supernatant, cells only, and supernatant only. The image in the center is a visual representation of the presented data.]]