Difference between revisions of "Part:BBa K842006"

 
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<partinfo>BBa_K842006 short</partinfo>
 
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Revision as of 21:20, 3 October 2012

cheA

CheA is a regulatory protein that becomes activated when the methyl-accepting chemotaxis protein (MCP) is not bound to any attractant. The activated cheA self-phosphorylates itself by hydrolyzing ATP in order to gain a phosphate group. CheA then donates its phosphate group to activate cheB or cheA.

Used for researcher control of signaling mechanisms that influence flagella function.

Cloning and Uses This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. It is to be used with other chemotaxis genes to form a complete regulatory pathway that controls the response system in MCP and the direction of rotation in flagella.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1286
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1286
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1286
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1286
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1286
    Illegal NgoMIV site found at 1636
  • 1000
    COMPATIBLE WITH RFC[1000]