Difference between revisions of "Part:BBa K901019"

Latest revision as of 21:05, 3 October 2012

PTA Expression Construct

Phosphotransacetylase (BBa_K901016) under the control of the LacI promoter (BBa_J04500)

Part of a 3 enzyme system designed to enhance the production of acetic acid by E. coli in the presence of high levels of sugars. AcK (BBa_K901015) and PTA (BBa_K901019) are enzymes that allow for the metabolism of acetic acid when there are low levels of available glucose. When glucose in the environment is high, these enzymes work in reverse as an overflow pathway for central metabolism, converting excess acetyl-CoA to acetate.

AatA (BBa_K901012) is an ABC transporter found in Acetobacter acetii which moves acetate out of the cell. This enzyme is introduced to drive the production of acetate forward and to confer resistance to high levels of acetic acid in the cellular environment.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 838
Illegal BamHI site found at 1603
Illegal BamHI site found at 1903
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1393
Illegal AgeI site found at 2281
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 Phosphotransacetylase ([https://parts.igem.org/Part:BBa_K901016 BBa_K901016]) under the control of the LacI promoter ([https://parts.igem.org/Part:BBa_J04500 BBa_J04500])
-Part of a 3 enzyme system designed to enhance the production of acetic acid by <i>E. coli</i> in the presence of high levels of sugars. ACK ([https://parts.igem.org/Part:BBa_K901015 BBa_K901015]) and PTA ([https://parts.igem.org/Part:BBa_K901019 BBa_K901019]) are enzymes that allow for the metabolism of acetic acid when there are low levels of available glucose. When glucose in the environment is high, these enzymes work in reverse as an overflow pathway for central metabolism, converting excess acetyl-CoA to acetate.
+Part of a 3 enzyme system designed to enhance the production of acetic acid by <i>E. coli</i> in the presence of high levels of sugars. AcK ([https://parts.igem.org/Part:BBa_K901015 BBa_K901015]) and PTA ([https://parts.igem.org/Part:BBa_K901019 BBa_K901019]) are enzymes that allow for the metabolism of acetic acid when there are low levels of available glucose. When glucose in the environment is high, these enzymes work in reverse as an overflow pathway for central metabolism, converting excess acetyl-CoA to acetate.
 AatA ([https://parts.igem.org/Part:BBa_K901012 BBa_K901012]) is an ABC transporter found in <i>Acetobacter acetii</i> which moves acetate out of the cell. This enzyme is introduced to drive the production of acetate forward and to confer resistance to high levels of acetic acid in the cellular environment.