Difference between revisions of "Part:BBa K726018"
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| − | <regulartext1> A device was constructed that contained the two enzymes VioD and VioC which on their own do not produce a pigment. | + | <regulartext1> A device was constructed that contained the two enzymes VioD and VioC which on their own do not produce a pigment. <br> |
<img align="center" style="margin-bottom:0px; width: 500px; margin-top:20px; padding:2; margin-left:55px;" src="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/violacein/VioDC.jpg"> | <img align="center" style="margin-bottom:0px; width: 500px; margin-top:20px; padding:2; margin-left:55px;" src="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/violacein/VioDC.jpg"> | ||
| − | <img align="center" style="margin-bottom:0px; width: | + | <img align="center" style="margin-bottom:0px; width: 200px; margin-top:20px; padding:2; margin-left:55px;" src="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/violacein/IMG_4881.jpg"> |
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| − | However, when cells containing this construct were grown together with strains containing | + | However, when cells containing this construct were grown together with strains containing our VioABE construct <a href="https://parts.igem.org/Part:BBa_K726016">BBa_K726016 </a>, a purple pigment (violacein) was produced. |
| + | More information on this process can be found on the <a href="http://2012.igem.org/Team:UCSF/Violacein_Results">2012 UCSF iGEM Violacein Results Page </a>. | ||
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Revision as of 20:56, 3 October 2012
T7Prom+HisTag+VioDC
This part contains a T7 promoter, lac operator, 6x his tag and the coding sequence for VioD and VioC (enzymes in the violacein production pathway).
Usage and Biology
Sequence and Features
Illegal BamHI site found at 123
Illegal SapI.rc site found at 1138