Difference between revisions of "Part:BBa K726018"

 
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<partinfo>BBa_K726018 short</partinfo>
 
<partinfo>BBa_K726018 short</partinfo>
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This part contains a T7 promoter, lac operator, 6x his tag and the coding sequence for VioD and VioC (enzymes in the violacein production pathway).  
 
This part contains a T7 promoter, lac operator, 6x his tag and the coding sequence for VioD and VioC (enzymes in the violacein production pathway).  
  
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===Usage and Biology===
 
===Usage and Biology===
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<html>
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<regulartext1> A device was constructed that contained the two enzymes VioD and VioC which on their own do not produce a pigment.
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<img align="center" style="margin-bottom:0px; width: 500px; margin-top:20px; padding:2; margin-left:55px;" src="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/violacein/VioDC.jpg">
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<img align="center" style="margin-bottom:0px; width: 500px; margin-top:20px; padding:2; margin-left:55px;" src="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/violacein/IMG_4881.jpg">
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<regulartext1>
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However, when cells containing this construct were grown together with strains containing the above construct (VioABE), a purple pigment (violacein) was produced.
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</html>
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 20:52, 3 October 2012

T7Prom+HisTag+VioDC

This part contains a T7 promoter, lac operator, 6x his tag and the coding sequence for VioD and VioC (enzymes in the violacein production pathway).


Usage and Biology

A device was constructed that contained the two enzymes VioD and VioC which on their own do not produce a pigment. However, when cells containing this construct were grown together with strains containing the above construct (VioABE), a purple pigment (violacein) was produced. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2571
    Illegal BamHI site found at 123
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1063
    Illegal SapI.rc site found at 1138