Difference between revisions of "Part:BBa K779607"
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<partinfo>BBa_K779607 short</partinfo> | <partinfo>BBa_K779607 short</partinfo> | ||
+ | This device consists of a modified TRE promoter with six tetO sites to provide low basal transcriptional activity, and the red fluroescent protein, mKate as a reporter. In the presence of rTTA3 and different concentrations of Doxycycline, we can modulate mKate expression. | ||
https://static.igem.org/mediawiki/2012/6/68/DoxCurveGraphPad.png | https://static.igem.org/mediawiki/2012/6/68/DoxCurveGraphPad.png |
Revision as of 20:49, 3 October 2012
Tre-Tight-mKate MammoBlock Device
This device consists of a modified TRE promoter with six tetO sites to provide low basal transcriptional activity, and the red fluroescent protein, mKate as a reporter. In the presence of rTTA3 and different concentrations of Doxycycline, we can modulate mKate expression.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 193
Illegal BsaI.rc site found at 859
Illegal SapI.rc site found at 241