Difference between revisions of "Part:BBa K824009:Experience"
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| − | We grew liquid cultures for 24 hours with varying concentrations of Cadmium Nitrate in the presence of our cells. After growth, the tests were spun down, then resuspended in 1X PBS (Phosphate Buffer Solution). We tested the detector with Cadmium since the promoter is responsive to multiple heavy metals. Due to safety issues, we were unable to use Arsenic because we are in | + | We grew liquid cultures for 24 hours with varying concentrations of Cadmium Nitrate in the presence of our cells. After growth, the tests were spun down, then resuspended in 1X PBS (Phosphate Buffer Solution). We tested the detector with Cadmium since the promoter is responsive to multiple heavy metals. Due to safety issues, we were unable to use Arsenic because we are in BSL1 and it is a known poison. The results were measured in fluorescence per OD 600. The graph below shows our results. |
<center>https://static.igem.org/mediawiki/2012/b/b7/Arsgraph.jpg</center> | <center>https://static.igem.org/mediawiki/2012/b/b7/Arsgraph.jpg</center> | ||
Latest revision as of 20:28, 3 October 2012
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K824009
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UNIQ0df3f95595d5e808-partinfo-00000000-QINU UNIQ0df3f95595d5e808-partinfo-00000001-QINU
We grew liquid cultures for 24 hours with varying concentrations of Cadmium Nitrate in the presence of our cells. After growth, the tests were spun down, then resuspended in 1X PBS (Phosphate Buffer Solution). We tested the detector with Cadmium since the promoter is responsive to multiple heavy metals. Due to safety issues, we were unable to use Arsenic because we are in BSL1 and it is a known poison. The results were measured in fluorescence per OD 600. The graph below shows our results.
