Difference between revisions of "Part:BBa K824009:Experience"
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| − | We grew liquid cultures for 24 hours with varying concentrations of Cadmium Nitrate in the presence of our cells. After growth, the tests were spun down, then resuspended in 1X PBS (Phosphate Buffer Solution). The results were measured in fluorescence per OD 600. The graph below shows our results. | + | We grew liquid cultures for 24 hours with varying concentrations of Cadmium Nitrate in the presence of our cells. After growth, the tests were spun down, then resuspended in 1X PBS (Phosphate Buffer Solution). We tested the detector with Cadmium since the promoter is responsive to multiple heavy metals. Due to safety issues, we were unable to use Arsenic because we are in BSL and it is a known poison. The results were measured in fluorescence per OD 600. The graph below shows our results. |
<center>https://static.igem.org/mediawiki/2012/b/b7/Arsgraph.jpg</center> | <center>https://static.igem.org/mediawiki/2012/b/b7/Arsgraph.jpg</center> | ||
Revision as of 20:28, 3 October 2012
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K824009
User Reviews
UNIQb24243333cbe556b-partinfo-00000000-QINU UNIQb24243333cbe556b-partinfo-00000001-QINU
We grew liquid cultures for 24 hours with varying concentrations of Cadmium Nitrate in the presence of our cells. After growth, the tests were spun down, then resuspended in 1X PBS (Phosphate Buffer Solution). We tested the detector with Cadmium since the promoter is responsive to multiple heavy metals. Due to safety issues, we were unable to use Arsenic because we are in BSL and it is a known poison. The results were measured in fluorescence per OD 600. The graph below shows our results.
