Difference between revisions of "Part:BBa J45005:Design"

(→‎References)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
changed the natural stop codon to TAA TAA
+
#no restriction enzyme cut sites in coding region
 +
#changed natural stop codon to TAA TAA
  
 
===Source===
 
===Source===

Revision as of 22:02, 8 June 2006


SAM:salicylic acid carboxyl methyltransferase; converts salicylic acid to methyl salicylate (winterg


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 652
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 232


Design Notes

  1. no restriction enzyme cut sites in coding region
  2. changed natural stop codon to TAA TAA

Source

  1. GenBank Accession No. AF133053
  2. Species: Clarkia Breweri

References

  1. corresponding Author: Eran Pichersky
  2. Pubmed: PMID: 10375393
  3. Abstract:
    • http://www.ncbi.nlm.nih.gov/entrezquery.fcgicmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10375393&query_hl=35&itool=pubmed_docsum
  4. Wiki Link:
    • http://openwetware.org/wiki/IGEM:MIT/2006/Notebook/2006-6-6