Difference between revisions of "Part:BBa K801065:Design"
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'''Source:'''<br> | '''Source:'''<br> | ||
| + | *BioBrick: BBa_I742111 | ||
<!--*Commercial system: plasmid name, system name, company name--> | <!--*Commercial system: plasmid name, system name, company name--> | ||
<!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?--> | <!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?--> | ||
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'''Organism:'''<br> | '''Organism:'''<br> | ||
| − | *Genesequence derived from '' | + | * Gene sequence derived from ''Citrus limon'' |
| + | <!--*Genesequence derived from ''?organism_name?''--> | ||
<!--*Codonoptimized for ''?organism_name?''--> | <!--*Codonoptimized for ''?organism_name?''--> | ||
<!--*Designed for the following Chassis: ''?organism-name?''--> | <!--*Designed for the following Chassis: ''?organism-name?''--> | ||
<!--*Statement about functionality in other chassis.--> | <!--*Statement about functionality in other chassis.--> | ||
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===References=== | ===References=== | ||
Revision as of 18:08, 3 October 2012
(+)-limonene synthase 1 with Strep-Tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1676
Illegal SapI.rc site found at 100
C-Terminal Strep-Tag added
See BBa_K801061
Keywords:
limonen, strep-tag
Abbreviations:
Design Notes
Related BioBrick:
- BBa_K801060: (+)-Limonene synthase 1 with Strep-tag and yeast consensus sequence
- BBa_K801061: (+)-Limonene synthase 1 coding region
- BBa_K801062: (+)-limonene synthase 1 expression cassette for yeast
- BBa_K801063: (+)-limonene synthase 1 expression cassette for yeast
- BBa_K801064: (+)-limonene synthase 1 expression cassette for yeast
- BBa_K801066: (+)-limonene synthase 1 with Yeast Consensus Sequence for improved expression
Quality control measures:
Backbone:
- Backbone name: pSB1C3'
Protein coding:
Enzymatic activity: EC-number 4.2.3.20
Cytotoxicity:
Safety notes:
Intellectual property:
Corresponding part author/authors:
Source
Source:
- BioBrick: BBa_I742111
Organism:
- Gene sequence derived from Citrus limon
References
Literature references:
- http://www.ncbi.nlm.nih.gov/pubmed/12084056 Lücker et al., 2002 Lücker, J., El Tamer, M. K., Schwab, W., Verstappen, F. W. A., van der Plas, L. H. W., Bouwmeester, H. J., and Verhoeven, H. A. (2002). Monoterpene biosynthesis in lemon (Citrus limon). cDNA isolation and functional analysis of four monoterpene synthases. Eur J Biochem, 269(13):3160–71.
Database references: