Difference between revisions of "Part:BBa K801064:Design"
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'''Source:'''<br> | '''Source:'''<br> | ||
| + | *BioBrick: BBa_I742111 | ||
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<!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?--> | <!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?--> | ||
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'''Organism:'''<br> | '''Organism:'''<br> | ||
| − | * | + | * Gene sequence derived from ''Citrus limon'' |
| + | <!--*Genesequence derived from ''?organism_name?''--> | ||
<!--*Codonoptimized for ''?organism_name?''--> | <!--*Codonoptimized for ''?organism_name?''--> | ||
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===References=== | ===References=== | ||
Revision as of 18:07, 3 October 2012
(+)-limonene synthase 1 expression cassette for yeast
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 138
Illegal AgeI site found at 2320 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 754
This is a composite part consisting of the yeast TEF2 promoter (BBa_K801010), the Citrus limon (+)-limonene synthase 1 coding region (BBa_K801061) and the yeast TEF1 terminator (BBa_K122003).
Keywords:
limonen, TEF1
Abbreviations:
Design Notes
Related BioBrick:
- BBa_K801060: (+)-Limonene synthase 1 with Strep-tag and yeast consensus sequence
- BBa_K801061: (+)-Limonene synthase 1 coding region
- BBa_K801062: (+)-limonene synthase 1 expression cassette for yeast
- BBa_K801063: (+)-limonene synthase 1 expression cassette for yeast
- BBa_K801065: (+)-limonene synthase 1 with Strep-Tag
- BBa_K801066: (+)-limonene synthase 1 with Yeast Consensus Sequence for improved expression
Quality control measures:
Backbone:
- Backbone name: pSB1C3'
Protein coding:
Enzymatic activity: EC-number 4.2.3.20
Cytotoxicity:
Safety notes:
Intellectual property:
Corresponding part author/authors:
Source
Source:
- BioBrick: BBa_I742111
Organism:
- Gene sequence derived from Citrus limon
References
Literature references:
- http://www.ncbi.nlm.nih.gov/pubmed/12084056 Lücker et al., 2002 Lücker, J., El Tamer, M. K., Schwab, W., Verstappen, F. W. A., van der Plas, L. H. W., Bouwmeester, H. J., and Verhoeven, H. A. (2002). Monoterpene biosynthesis in lemon (Citrus limon). cDNA isolation and functional analysis of four monoterpene synthases. Eur J Biochem, 269(13):3160–71.
Database references: