Difference between revisions of "Part:BBa K892403"
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+ | [[Image:UWashington HB36cytomutant.jpg|border|800px|center|thumb|HB36.5 flu binder tested using Yeast surface display at 100 nM H2 on the left, H312A mutant tested on the right at 100 nM H2 on the right. HB36.5 Shows little to no binding at this HA concentration. H312A dramatically increases binding to H2.]] | ||
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===Usage and Biology=== | ===Usage and Biology=== |
Latest revision as of 17:15, 3 October 2012
HB36.5_H312A
A mutated HB80.4 flu binder aimed to increase binding to H2, a subtype of hemagglutinin on the surface of the Influenza virus. This mutant has a point mutation at residue 312 from Histidine to Alanine, which dramatically increases the binding to H2 over the native HB36.5 binder.
Usage and Biology
This part was tested by the [http://2012.igem.org/Team:Washington 2012 University of Washington] iGEM team to bind to various subtypes of Hemagglutinin in Group 1.To test BBa _K892401, it was inserted into a protein expression vector, pETCON. HB36.5_H312A was then produced and transformed into yeast as described in the [http://2012.igem.org/Team:Washington/Protocols/Yeast 2012 Team's Yeast Transformation Protocol]. The binding protein was then tested for activity against H2. For a detailed description of the methodology, please see the [http://2012.igem.org/Team:Washington/Protocols/Display 2012 UW iGEM Yeast Surface Display protocol]. The resulting data is shown below.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 107
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 107
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 289
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 107
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 107
- 1000COMPATIBLE WITH RFC[1000]