Difference between revisions of "Part:BBa K892410"

 
 
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<partinfo>BBa_K892410 short</partinfo>
 
<partinfo>BBa_K892410 short</partinfo>
  
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Designed Hemagglutanin binding protein
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A mutated HB36.5 flu binder aimed to increase binding to H2, a subtype of hemagglutinin on the surface of the Influenza virus. This mutant has a point mutation at residue 317 from Phenylalanine to Serine
  
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===Usage and Biology===
 
===Usage and Biology===
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This part was tested by the [http://2012.igem.org/Team:Washington 2012 University of Washington] iGEM team to bind to various subtypes of Hemagglutinin in Group 1.To test BBa _K892401, it was inserted into a protein expression vector, pETCON. HB36.5_F317H was then produced and transformed into yeast as described in the [http://2012.igem.org/Team:Washington/Protocols/Yeast  2012 Team's Yeast Transformation Protocol]. The binding protein was then tested for activity against H2. For a detailed description of the methodology, please see the [http://2012.igem.org/Team:Washington/Protocols/Display 2012 UW iGEM Yeast Surface Display protocol]. The resulting data is shown below.
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[[Image:UWashington FLU HB36 results graph.png|border|800px|center|thumb|HB36.5 and its variants tested against hemagglutinin subtype 2. HB36.5 is shown to have very little activity in binding H2.]]
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K892410 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K892402 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K892410 parameters</partinfo>
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<partinfo>BBa_K892402 parameters</partinfo>
 
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Latest revision as of 16:37, 3 October 2012

HB36.5 F317H

A mutated HB36.5 flu binder aimed to increase binding to H2, a subtype of hemagglutinin on the surface of the Influenza virus. This mutant has a point mutation at residue 317 from Phenylalanine to Serine

Usage and Biology

This part was tested by the [http://2012.igem.org/Team:Washington 2012 University of Washington] iGEM team to bind to various subtypes of Hemagglutinin in Group 1.To test BBa _K892401, it was inserted into a protein expression vector, pETCON. HB36.5_F317H was then produced and transformed into yeast as described in the [http://2012.igem.org/Team:Washington/Protocols/Yeast 2012 Team's Yeast Transformation Protocol]. The binding protein was then tested for activity against H2. For a detailed description of the methodology, please see the [http://2012.igem.org/Team:Washington/Protocols/Display 2012 UW iGEM Yeast Surface Display protocol]. The resulting data is shown below.

HB36.5 and its variants tested against hemagglutinin subtype 2. HB36.5 is shown to have very little activity in binding H2.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 107
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 107
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 289
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 107
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 107
  • 1000
    COMPATIBLE WITH RFC[1000]