Difference between revisions of "Part:BBa K892401"

 
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<partinfo>BBa_K892401 short</partinfo>
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===HB36.5 Flu binding protein===
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A flu binding protein originally shown to binding H1, a subtype of Hemagglutinin on the surface of the Influenza virus. This was originally identified and tested by both Fleishmen et al, and Whitehead et al., respectively.
  
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A designed protein that binds hemagglutinin.
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===Usage and Biology===
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This part was tested by the 2012 University of Washington iGEM team to bind to various subtypes of Hemagglutinin in Group 1.To test BBa _K892401, it was inserted into a protein expression vector, pETCON. HB36.5 was then produced and transformed into yeast as described in the 2012 iGEM Team's Yeast Transformation Protocol. The binding protein was then tested for activity against H2. For a detailed description of the methodology, please see the 2012 UW iGEM Yeast Surface Display protocol. The resulting data is shown below.
  
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===Usage and Biology===
 
  
 
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Revision as of 16:01, 3 October 2012

HB36.5 Flu binding protein

A flu binding protein originally shown to binding H1, a subtype of Hemagglutinin on the surface of the Influenza virus. This was originally identified and tested by both Fleishmen et al, and Whitehead et al., respectively.

Usage and Biology

This part was tested by the 2012 University of Washington iGEM team to bind to various subtypes of Hemagglutinin in Group 1.To test BBa _K892401, it was inserted into a protein expression vector, pETCON. HB36.5 was then produced and transformed into yeast as described in the 2012 iGEM Team's Yeast Transformation Protocol. The binding protein was then tested for activity against H2. For a detailed description of the methodology, please see the 2012 UW iGEM Yeast Surface Display protocol. The resulting data is shown below.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 107
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 107
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 289
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 107
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 107
  • 1000
    COMPATIBLE WITH RFC[1000]