Difference between revisions of "Part:BBa K766001:Design"
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Theere is something wrong with Part:BBa_J09855. So we modify the promoter and correct the sequence. Besides, we add RFP downstream of Plux promoter as a reporter. | Theere is something wrong with Part:BBa_J09855. So we modify the promoter and correct the sequence. Besides, we add RFP downstream of Plux promoter as a reporter. | ||
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| + | Thank iGEM2006_PennState! | ||
===References=== | ===References=== | ||
Latest revision as of 15:29, 3 October 2012
C6HSL reporter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 987
Design Notes
There is something wrong with the previous part BBa_J09855, which doesn't work well. After we sequence the part, we find that the sequence of Plac promoter and Plux promoter is incorrect. So that we rebuild this part using overlap PCR and DNA systhesis. Finally, our part can work normally.
Source
Theere is something wrong with Part:BBa_J09855. So we modify the promoter and correct the sequence. Besides, we add RFP downstream of Plux promoter as a reporter.
Thank iGEM2006_PennState!