Difference between revisions of "Part:BBa K801063:Design"

(Design Notes)
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<br>'''Keywords:'''
 
<br>'''Keywords:'''
 
<!--These keywords are necessary to find your part using a fulltext sarch.-->
 
<!--These keywords are necessary to find your part using a fulltext sarch.-->
<!--keyword_1, keyword_2, keyword_3, keyword_4, keyword_5-->
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limonen
  
 
<br>'''Abbreviations:'''
 
<br>'''Abbreviations:'''
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'''Backbone:'''<br>
 
'''Backbone:'''<br>
<!--*Backbone name: pSB1C3'/?backbone_name?-->
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*Backbone name: pSB1C3'
 
<!--*Resistance: Amp/Cp/Kan/Tet-->
 
<!--*Resistance: Amp/Cp/Kan/Tet-->
 
<!--*Copynumber: low/medium/high-->
 
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'''Enzymatic activity:'''
 
'''Enzymatic activity:'''
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EC-number 4.2.3.20
  
 
'''Cytotoxicity:'''<br>
 
'''Cytotoxicity:'''<br>
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'''Organism:'''<br>
 
'''Organism:'''<br>
<!--*Genesequence derived from ''?organism_name?''-->
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*Genesequence derived from ''citrus limon''
 
<!--*Codonoptimized for ''?organism_name?''-->
 
<!--*Codonoptimized for ''?organism_name?''-->
 
<!--*Designed for the following Chassis: ''?organism-name?''-->
 
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Revision as of 15:28, 3 October 2012

(+)-limonene synthase 1 expression cassette for yeast


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2127
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 211
    Illegal SapI.rc site found at 561


This is a composite part consisting of the yeast TEF1 promoter (BBa_K319003), the Citrus limon (+)-limonene synthase 1 coding region (BBa_K801061) and the yeast TEF1 terminator (BBa_K801011).


Keywords: limonen


Abbreviations:

Design Notes

Related BioBrick:

Quality control measures:

Backbone:

  • Backbone name: pSB1C3'

Protein coding:

Enzymatic activity: EC-number 4.2.3.20

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:


Organism:

  • Genesequence derived from citrus limon


References

Literature references:

Database references: