Difference between revisions of "Part:BBa K801041:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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'''Related BioBrick:'''
 
'''Related BioBrick:'''
 
<!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
 
<!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
−
*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_K801030 BBa_K801030:Polypeptide containing SV40 nuclear localization sequence ], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K801030 BBa_K801030: Name_of_part], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K365005 BBa_K365005: Name_of_part], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K801033 BBa_K801033: Name_of_part]
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*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_K801030 BBa_K801030:Polypeptide containing SV40 nuclear localization sequence ], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K801030 BBa_K801030: Polypeptide containing SV40 nuclear localization sequence], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K365005 BBa_K365005:20 aa linker RFC-25 ], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K801033 BBa_K801033: LexA DNA binding protein]
  
 
'''Cloning details:'''<br>
 
'''Cloning details:'''<br>

Latest revision as of 15:10, 3 October 2012

SV40NLS-PhyB-linker-LexA (part for a LexA based light-switchable promoter system)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 550
    Illegal BglII site found at 3390
    Illegal BamHI site found at 632
    Illegal XhoI site found at 583
    Illegal XhoI site found at 602
    Illegal XhoI site found at 2737
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2122
    Illegal BsaI site found at 2528
    Illegal SapI site found at 799


Composite part of BBa_K801030, BBa_K801030, BBa_K365005, BBa_K801033


Keywords: GAL4, PIF3, ligt switchable
Abbreviations:

Design Notes

Related BioBrick:

Cloning details:

  • Designed in RFC25

Quality control measures:

Backbone:

  • Backbone name: pSB1C3

Protein coding:

Enzymatic activity:

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:


Organism:


References

Literature references:

Database references: