Difference between revisions of "Part:BBa P1010"
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Most BioBrick plasmids are delivered with the P1010 insert | Most BioBrick plasmids are delivered with the P1010 insert | ||
| − | see [https://parts.igem.org/cgi/partsdb/dna.cgi?part_name=P1010 Physical DNA] for the current list of plasmids that are available in this form. | + | see [https://parts.igem.org/cgi/partsdb/dna.cgi?part_name=P1010 P1010 Physical DNA] for the current list of plasmids that are available in this form. |
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_P1010 SequenceAndFeatures</partinfo> | <partinfo>BBa_P1010 SequenceAndFeatures</partinfo> | ||
Revision as of 21:20, 8 June 2006
ccdB cell death gene
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The CcdB protein, constitutively expressed by P1010, is lethal to most of the BioBrick cell strains, only DB3.1 is resistant. |
P1010 is used when putting BioBrick parts into BioBrick plasmids. The part to be inserted and the plasmid are cut with BioBrick enzymes and mixed. The mixture will include both the original uncut or religated plasmid and the desired structure. However, because of CcdB, all of the cells containing the original plasmid die.
BioBrick Plasmids Containing This Part
Most BioBrick plasmids are delivered with the P1010 insert see P1010 Physical DNA for the current list of plasmids that are available in this form.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 664
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 114