Difference between revisions of "Part:BBa K801073:Design"

Revision as of 12:40, 3 October 2012

CaXMT1 expression cassette for yeast

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 138
1000
COMPATIBLE WITH RFC[1000]

Composite part of BBa_K801010, BBa_K801070, BBa_K801012

Keywords: CaMXMT1, TEF2 promoter, ADH1 terminator, caffeine, caffeine pathway, caffeine synthesis pathway

Abbreviations:

CaXMT1 = xanthosine methyltransferase

Design Notes

Related BioBrick:

Cloning details:

Quality control measures:

Part was totally sequenced

Backbone:

Backbone name: pSB1C3

Protein coding:

Enzymatic activity:

Cytotoxicity:
This Biobrick is one part of the pathway, which leads to caffeine (the BioBricks BBa_K801074 and BBa_K801075 are further needed). We investigated the toxicity of caffeine and found out, that the growth of yeast strains was inhibited at concentrations of 10 mM. Caffeine with a concentration of 100 mM even showed a lethal effect on yeast cells. Lower concentrations of caffeine (range of micro molar) did not show any growth inhibition or effect.

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:

Organism:

References

Literature references:

Database references:

@@ Line 37: / Line 37: @@
 <!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
 <!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
-<!--*Part was partly sequenced/Part was totally sequenced-->
+*Part was totally sequenced
 '''Backbone:'''<br>
-<!--*Backbone name: pSB1C3'/?backbone_name?-->
+*Backbone name: pSB1C3
 <!--*Resistance: Amp/Cp/Kan/Tet-->
 <!--*Copynumber: low/medium/high-->