Difference between revisions of "Part:BBa K801060:Design"

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limonene synthase, limonene
 
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Revision as of 12:40, 3 October 2012

(+)-Limonene synthase 1 with Strep-tag and yeast consensus sequence.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1683
    Illegal SapI.rc site found at 107


Keywords: limonene synthase, limonene


Abbreviations:

Design Notes

Related BioBrick:

Quality control measures:

Backbone:

Protein coding:

Enzymatic activity:

Cytotoxicity:
This Biobrick leads to the production of limonene. We investigated the toxicity of limonene and found out, that the growth of yeast strains was inhibited at concentrations of 1 mM. Limonene with a concentration of 100 mM even showed a lethal effect. Lower concentrations of limonene (range of micro molar) did not show any growth inhibition or effect.

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:


Organism:


References

Literature references:

Database references:

  • [http://www.ncbi.nlm.nih.gov/nuccore/21435702 GenBank: source of the sequence]