Difference between revisions of "Part:BBa K801073:Design"

(→‎Design Notes)
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Composite part of BBa_K801010, BBa_K801070, BBa_K801012
 
Composite part of BBa_K801010, BBa_K801070, BBa_K801012
  
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<br>'''Keywords:'''
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<br>'''Keywords:''' CaMXMT1, TEF2 promoter, ADH1 terminator, caffeine, caffeine pathway, caffeine synthesis pathway
 
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<br>'''Abbreviations:'''
 
<br>'''Abbreviations:'''
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<!--*used_abbreviation_1 = full_name_of_used_abbreviations_1-->
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*CaXMT1 = xanthosine methyltransferase
 
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'''Related BioBrick:'''
 
'''Related BioBrick:'''
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<!--*Other versions:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part] -->
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*Other versions:
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<!--*Related BioBricks:[https://parts.igem.org/wiki/index.php?title=Part:BBa_?????? BBa_??????: Name_of_part]
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**[https://parts.igem.org/Part:BBa_K801070 BBa_K801070: xanthosine methyltransferase CaXMT1-strep]
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*Related BioBricks:
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** [https://parts.igem.org/Part:BBa_K801071 BBa_K801071: 7-methylxanthine N-methyltransferase CaMXMT1-strep]
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** [https://parts.igem.org/Part:BBa_K801072 BBa_K801072: 3,7-dimethylxanthine N-methyltransferase CaDXMT1-strep]
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** [https://parts.igem.org/Part:BBa_K801073 BBa_K801073: CaXMT1 expression cassette for yeast]
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** [https://parts.igem.org/Part:BBa_K801074 BBa_K801074: CaMXMT1 expression cassette for yeast]
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** [https://parts.igem.org/Part:BBa_K801075 BBa_K801075: CaDXMT1 expression cassette for yeast]
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** [https://parts.igem.org/Part:BBa_K801076 BBa_K801076: Yeast expression cassette for CaXMT1 and CaMXMT1]
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** [https://parts.igem.org/Part:BBa_K801077 BBa_K801077: Caffeine Synthesis Pathway pTEF2-XMT1-tADH1-pTEF1-MXMT1-tADH1-pTEF2-DXMT1-tADH1]
  
 
'''Cloning details:'''<br>
 
'''Cloning details:'''<br>

Revision as of 12:37, 3 October 2012

CaXMT1 expression cassette for yeast


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 138
  • 1000
    COMPATIBLE WITH RFC[1000]


Composite part of BBa_K801010, BBa_K801070, BBa_K801012


Keywords: CaMXMT1, TEF2 promoter, ADH1 terminator, caffeine, caffeine pathway, caffeine synthesis pathway


Abbreviations:

  • CaXMT1 = xanthosine methyltransferase

Design Notes

Related BioBrick:

Cloning details:

Quality control measures:

Backbone:

Protein coding:

Enzymatic activity:

Cytotoxicity:
This Biobrick is one part of the pathway, which leads to caffeine (the BioBricks BBa_K801074 and BBa_K801075 are further needed). We investigated the toxicity of caffeine and found out, that the growth of yeast strains was inhibited at concentrations of 10 mM. Caffeine with a concentration of 100 mM even showed a lethal effect on yeast cells. Lower concentrations of caffeine (range of micro molar) did not show any growth inhibition or effect.

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:


Organism:


References

Literature references:

Database references: