Difference between revisions of "Part:BBa K801073"
Line 4: Line 4:
 
Yeast expression cassette for CaXMT1 ([https://parts.igem.org/Part:BBa_K801070 BBa_K801070]) controlled by the yeast TEF2 promoter ([https://parts.igem.org/Part:BBa_K801010 BBa_K801010]) and the yeast ADH1 terminator ([https://parts.igem.org/Part:BBa_K801012 BBa_K801012]).
 
Yeast expression cassette for CaXMT1 ([https://parts.igem.org/Part:BBa_K801070 BBa_K801070]) controlled by the yeast TEF2 promoter ([https://parts.igem.org/Part:BBa_K801010 BBa_K801010]) and the yeast ADH1 terminator ([https://parts.igem.org/Part:BBa_K801012 BBa_K801012]).
  
−
This BioBrick is the generator for the enzyme xanthosine N-methyltransferase 1 (CaXMT1) and part of the caffeine synthesis pathway. It is regulated by the constitutive promoter Tef2, which is a strong yeast promoter. The used terminator Adh1, is a widely used yeast terminator. The Tef2 promoter was prefered compared to the Tef1 promoter (which is even stronger) in order to limit metabolic stress, which could result in a positive selection of natural mutants (after genome integration).
+
This BioBrick is the generator for the enzyme xanthosine N-methyltransferase 1 (CaXMT1) and part of the caffeine synthesis pathway. For creating an expression cassette with all three enzymes of the caffeine synthesis pathway based on the substrate Xanthosine [https://parts.igem.org/Part:BBa_K801077 BBa_K801077] different promoters and terminators were assembled to each enzyme. CaXMT1 is regulated by the constitutive promoter Tef2, which is a strong yeast promoter. The used terminator Adh1, is a widely used yeast terminator. The Tef2 promoter was prefered compared to the Tef1 promoter (which is even stronger) in order to limit metabolic stress, which could result in a positive selection of natural mutants (after genome integration).
  
  

Revision as of 11:58, 3 October 2012

CaXMT1 expression cassette for yeast

Yeast expression cassette for CaXMT1 (BBa_K801070) controlled by the yeast TEF2 promoter (BBa_K801010) and the yeast ADH1 terminator (BBa_K801012).

This BioBrick is the generator for the enzyme xanthosine N-methyltransferase 1 (CaXMT1) and part of the caffeine synthesis pathway. For creating an expression cassette with all three enzymes of the caffeine synthesis pathway based on the substrate Xanthosine BBa_K801077 different promoters and terminators were assembled to each enzyme. CaXMT1 is regulated by the constitutive promoter Tef2, which is a strong yeast promoter. The used terminator Adh1, is a widely used yeast terminator. The Tef2 promoter was prefered compared to the Tef1 promoter (which is even stronger) in order to limit metabolic stress, which could result in a positive selection of natural mutants (after genome integration).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 138
  • 1000
    COMPATIBLE WITH RFC[1000]