Difference between revisions of "Part:BBa K801097:Design"

Revision as of 11:22, 3 October 2012

Chalcone O-methyltransferase (OMT1) + yeast consensus sequence

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 485
Illegal XhoI site found at 300
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

http://www.ncbi.nlm.nih.gov/nucleotide/167613934/

Keywords: Coumaryol-CoA, Coumaroyl-Coenzyme A, Xanthohumol, Biosynthesis, aromatic prenyltransferase

Abbreviations: OMT = O-methyltransferase

Design Notes

Related BioBrick:

Other versions: BBa_K801098: OMT1 coding region

Cloning details:
Designed in RFC10/RFC25

Quality control measures:
Part was totally sequenced

Backbone:

Backbone name: pSB1C3
Resistance: Cp
Copynumber: high

Protein coding:

Protein: O-methyltransferase
Tag: c-terminally fused Strep

Enzymatic activity:
EC-number: EC 2.1.1

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:
Plasmid with OMT provided by Jonathan E. Page, National Research Council Canada, Plant Biotechnology Institute, Canada

Organism:
Genesequence derived from Humulus lupulus

References

Literature references:
[http://www.ncbi.nlm.nih.gov/pubmed/18223037 Pubmed : Nagel, J., Culley, L.K., Lu, Y., Liu, E., Matthews, P.D., Stevens, J.F., Page, J.E., 2008. EST analysis of hop glandular trichomes identifies an O-methyltransferase that catalyzes the biosynthesis of xanthohumol. Plant Cell 20, 186–200]

Database references:
[http://www.ncbi.nlm.nih.gov/nuccore/EU309725.1 GenBank: Humulus lupulus O-methyltransferase 1 (OMT1) mRNA, complete cds]

[http://www.uniprot.org/uniprot/B0ZB55 Uniprot: O-methyltransferase 1]

@@ Line 34: / Line 34: @@
 '''Backbone:'''<br>
-Backbone name: pSB1C3
+*Backbone name: pSB1C3
-Resistance: Cp
+*Resistance: Cp
-<!--*Copynumber: low/medium/high-->
+*Copynumber: high
 '''Protein coding:'''<br>