Difference between revisions of "Part:BBa K801094:Design"

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'''Protein coding:'''<br>
 
'''Protein coding:'''<br>
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Protein: naringenin-chalcone synthase
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*Protein: naringenin-chalcone synthase
 
<!--*The protein has the amino acid replacements ???99??? to ???99???.-->
 
<!--*The protein has the amino acid replacements ???99??? to ???99???.-->
 
<!--*The protein encoded is posttranslationally modified by ???.-->
 
<!--*The protein encoded is posttranslationally modified by ???.-->
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<!--*Tag: n-terminally fused/c-terminally fused His5/His6/Strep/Flag/other-->
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*Tag: c-terminally fused Strep
  
 
'''Enzymatic activity:'''<br>
 
'''Enzymatic activity:'''<br>

Revision as of 09:52, 3 October 2012

Naringenin - chalcone synthase (CHS) + yeast consensus sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 723
    Illegal BsaI.rc site found at 1144
    Illegal SapI.rc site found at 846



Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA

http://www.ncbi.nlm.nih.gov/nucleotide/11096318/

PMID: 15932991


Keywords: Coumaryol-CoA, Coumaroyl-Coenzyme A, Xanthohumol, Biosynthesis, 4-coumarate-coenzyme A ligase, aromatic prenyltransferase


Abbreviations: CHS = naringenin-chalcone synthase

Design Notes

Related BioBrick:

Other versions: BBa_K801095: CHS coding region

Related BioBricks: BBa_K801090, BBa_K801091, BBa_K801092, BBa_K801093, BBa_K801095, BBa_K801096, BBa_K801097, BBa_K801098

Cloning details:
Designed in RFC10/RFC25

Quality control measures:
Part was totally sequenced

Backbone:
Backbone name: pSB1C3 Resistance: Cp

Protein coding:

  • Protein: naringenin-chalcone synthase
  • Tag: c-terminally fused Strep

Enzymatic activity:
EC-number: 2.3.1.74

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:
Plasmid: pKS2µHyg-PAL-4CL-CHS, provided by John A. Morgan, Purdue University, USA


Organism:
Genesequence derived from Hypericum androsaemum

References

Literature references:
[http://www.ncbi.nlm.nih.gov/pubmed/15932991 Pubmed : Jiang, H., Wood, K.V., Morgan, J.A., 2005. Metabolic Engineering of the Phenylpropanoid Pathway in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 71, 2962–2969]


Database references:
[http://www.ncbi.nlm.nih.gov/nuccore/AF315345.1 GenBank: Hypericum androsaemum chalcone synthase mRNA, complete cds]

[http://www.uniprot.org/uniprot/Q9FUB7 Uniprot: Chalcone synthase]