Difference between revisions of "Part:BBa K801096:Design"
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'''Related BioBrick:''' | '''Related BioBrick:''' | ||
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− | + | Related BioBricks: [https://parts.igem.org/Part:BBa_K801090 BBa_K801090], [https://parts.igem.org/Part:BBa_K801091 BBa_K801091], [https://parts.igem.org/Part:BBa_K801092 BBa_K801092], [https://parts.igem.org/Part:BBa_K801093 BBa_K801093], [https://parts.igem.org/Part:BBa_K801094 BBa_K801094], [https://parts.igem.org/Part:BBa_K801095 BBa_K801095], [https://parts.igem.org/Part:BBa_K801097 BBa_K801097], [https://parts.igem.org/Part:BBa_K801098 BBa_K801098] | |
'''Cloning details:'''<br> | '''Cloning details:'''<br> | ||
− | + | Designed in RFC10/RFC25 | |
<!--*Mutation C889G to delete XbaI restriction site--> | <!--*Mutation C889G to delete XbaI restriction site--> | ||
<!--*Truncation upstream/downstream compared to template, ?explanation?--> | <!--*Truncation upstream/downstream compared to template, ?explanation?--> |
Revision as of 09:22, 3 October 2012
Aromatic prenyltransferase (APT) coding region
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Synthesized by Geneart, yeast codon optimized
http://www.ncbi.nlm.nih.gov/nuccore/AB543053
Keywords:
Coumaryol-CoA, Coumaroyl-Coenzyme A, Xanthohumol, Biosynthesis, naringenin-chalcone synthase, O-methyltransferase 1
Abbreviations:
APT = aromatic prenyltransferase
Design Notes
Related BioBrick:
Related BioBricks: BBa_K801090, BBa_K801091, BBa_K801092, BBa_K801093, BBa_K801094, BBa_K801095, BBa_K801097, BBa_K801098
Cloning details:
Designed in RFC10/RFC25
Quality control measures:
Part was totally sequenced
Backbone:
Backbone name: pSB1C3
Resistance: Cp
Protein coding:
Protein: aromatic prenyltransferase
Enzymatic activity:
EC-number: 2.5.1
Cytotoxicity:
Safety notes:
Intellectual property:
Corresponding part author/authors:
Source
Source:
Synthesized by Geneart
Organism:
Genesequence derived from Humulus lupulus
Codonoptimized for Saccharomyces cerevisiae
References
Literature references:
Database references:
[http://www.ncbi.nlm.nih.gov/nuccore/AB543053.1 GenBank: Humulus lupulus mRNA for aromatic prenyltransferase, complete cds]
[http://www.uniprot.org/uniprot/E5RP65 Uniprot: Aromatic prenyltransferase]