Difference between revisions of "Part:BBa K801094:Design"

Revision as of 09:15, 3 October 2012

Naringenin - chalcone synthase (CHS) + yeast consensus sequence

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 723
Illegal BsaI.rc site found at 1144
Illegal SapI.rc site found at 846

Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA

http://www.ncbi.nlm.nih.gov/nucleotide/11096318/

PMID: 15932991

Keywords: Coumaryol-CoA, Coumaroyl-Coenzyme A, Xanthohumol, Biosynthesis, 4-coumarate-coenzyme A ligase, aromatic prenyltransferase

Abbreviations: CHS = naringenin-chalcone synthase

Design Notes

Related BioBrick:

Other versions: BBa_K801095: CHS coding region

Cloning details:
Designed in RFC25

Quality control measures:
Part was totally sequenced

Backbone:
Backbone name: pSB1C3 Resistance: Cp

Protein coding:
Protein: naringenin-chalcone synthase

Enzymatic activity:
EC-number: 2.3.1.74

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:
Plasmid: pKS2µHyg-PAL-4CL-CHS, provided by John A. Morgan, Purdue University, USA

Organism:
Genesequence derived from Hypericum androsaemum

References

Literature references:
[http://www.ncbi.nlm.nih.gov/pubmed/15932991 Pubmed : Jiang, H., Wood, K.V., Morgan, J.A., 2005. Metabolic Engineering of the Phenylpropanoid Pathway in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 71, 2962–2969]

Database references:
[http://www.ncbi.nlm.nih.gov/nuccore/AF315345.1 GenBank: Hypericum androsaemum chalcone synthase mRNA, complete cds]

[http://www.uniprot.org/uniprot/Q9FUB7 Uniprot: Chalcone synthase]

@@ Line 23: / Line 23: @@
 '''Related BioBrick:'''
-Other versions: [https://parts.igem.org/Part:BBa_K801095 BBa_K80109: CHS coding region ]
+Other versions: [https://parts.igem.org/Part:BBa_K801095 BBa_K801095: CHS coding region ]
 Related BioBricks: [https://parts.igem.org/Part:BBa_K801090 BBa_K801090], [https://parts.igem.org/Part:BBa_K801091 BBa_K801091], [https://parts.igem.org/Part:BBa_K801092 BBa_K801092], [https://parts.igem.org/Part:BBa_K801093 BBa_K801093], [https://parts.igem.org/Part:BBa_K801095 BBa_K801095], [https://parts.igem.org/Part:BBa_K801096 BBa_K801096], [https://parts.igem.org/Part:BBa_K801097 BBa_K801097], [https://parts.igem.org/Part:BBa_K801098 BBa_K801098]