Difference between revisions of "Part:BBa P1010"

Revision as of 20:39, 8 June 2006

ccdB cell death gene

The CcdB protein, constitutively expressed by P1010, is lethal to most of the BioBrick cell strains, only DB3.1 is resistant.

P1010 is used when putting BioBrick parts into BioBrick plasmids. Most BioBrick plasmids are delivered with the P1010 insert see Part:BBaq_P1010:Physical_DNA P1010 Physical DNA for the current list of plasmids that are available in this form.

BioBrick Plasmids Containing This Part

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 664
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 114

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	P1010 is used when putting BioBrick parts into BioBrick plasmids. Most BioBrick plasmids are delivered with the P1010 insert		P1010 is used when putting BioBrick parts into BioBrick plasmids. Most BioBrick plasmids are delivered with the P1010 insert
−	see [Part:BBaq_P1010:Physical_DNA P1010 Physical DNA] for the current list of plasmids that are available in this form.	+	see [[Part:BBaq_P1010:Physical_DNA P1010 Physical DNA]] for the current list of plasmids that are available in this form.