Difference between revisions of "Part:BBa P1010"
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[[Image:CcdB_Plasmid.png]]
 
[[Image:CcdB_Plasmid.png]]
 
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The CcdB protein, expressed by P1010, is lethal to most of the BioBrick cell strains, only DB3.1 is resistant.
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The CcdB protein, constitutively expressed by P1010, is lethal to most of the BioBrick cell strains, only DB3.1 is resistant.
 
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P1010 is used to put BioBrick parts
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P1010 is used when putting BioBrick parts into BioBrick plasmids. Most BioBrick plasmids are delivered with the P1010 insert
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see [Part:BBaq_P1010:Physical_DNA P1010 Physical DNA] for the current list of plasmids that are available in this form.
  
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aid the process of moving a BioBrick part to a new plasmid. A target plasmid carrying P1010 is cut and mixed with the cut insert. Plasmids that recombine are selected against because they kill the cell that picks them up.
 
  
 
===BioBrick Plasmids Containing This Part===
 
===BioBrick Plasmids Containing This Part===

Revision as of 20:39, 8 June 2006

ccdB cell death gene

CcdB Plasmid.png

The CcdB protein, constitutively expressed by P1010, is lethal to most of the BioBrick cell strains, only DB3.1 is resistant.

P1010 is used when putting BioBrick parts into BioBrick plasmids. Most BioBrick plasmids are delivered with the P1010 insert see [Part:BBaq_P1010:Physical_DNA P1010 Physical DNA] for the current list of plasmids that are available in this form.


BioBrick Plasmids Containing This Part

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 664
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 114