Difference between revisions of "Part:BBa K801090:Design"

Revision as of 08:52, 3 October 2012

phenylalanine ammonia lyase (PAL) + yeast consensus sequence

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2096
Illegal BamHI site found at 358
Illegal XhoI site found at 409
Illegal XhoI site found at 472
Illegal XhoI site found at 490
Illegal XhoI site found at 568
Illegal XhoI site found at 769
Illegal XhoI site found at 1012
Illegal XhoI site found at 1759
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 928
Illegal NgoMIV site found at 1174
Illegal NgoMIV site found at 1342
Illegal NgoMIV site found at 1485
Illegal NgoMIV site found at 1819
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 469
Illegal BsaI site found at 799
Illegal BsaI site found at 805
Illegal BsaI.rc site found at 1930
Illegal SapI site found at 759

Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA

http://www.ncbi.nlm.nih.gov/nuccore/AX366866

PMID: 15932991

Keywords: Coumaryol-CoA, Coumaroyl-Coenzyme A, Xanthohumol, Biosynthesis, 4-coumarate-coenzyme A ligase

Abbreviations: PAL = Phenylalanine Ammonia Lyase

Design Notes

Related BioBrick:

Other versions:BBa_K801091: PAL coding region

Cloning details:

Quality control measures:
Part was totally sequenced

Backbone:
Backbone name: pSB1C3 Resistance: Cp

Protein coding:
Protein: Phenylalanine Ammonia Lyase

Enzymatic activity: EC-number 4.3.1.25

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:
Plasmid: pKS2µHyg-PAL-4CL-CHS, provided by John A. Morgan, Purdue University, USA

Organism:
Genesequence derived from Rhodosporidium toruloides'

References

Literature references:
[http://www.ncbi.nlm.nih.gov/pubmed/15932991 Pubmed : Jiang, H., Wood, K.V., Morgan, J.A., 2005. Metabolic Engineering of the Phenylpropanoid Pathway in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 71, 2962–2969]

Database references:
[http://www.ncbi.nlm.nih.gov/nuccore/AX366866 GenBank: Sequence 18 from Patent WO0208402]

[http://www.uniprot.org/uniprot/P11544 Uniprot: Phenylalanine/tyrosine ammonia-lyase]

@@ Line 24: / Line 24: @@
 '''Related BioBrick:'''
 Other versions:[https://parts.igem.org/Part:BBa_K801091 BBa_K801091: PAL coding region]
-Related BioBricks:[https://parts.igem.org/Part:BBa_K801091 BBa_K801091], [https://parts.igem.org/Part:BBa_K801092 BBa_K801092]
+Related BioBricks:[https://parts.igem.org/Part:BBa_K801091 BBa_K801091], [https://parts.igem.org/Part:BBa_K801092 BBa_K801092], [https://parts.igem.org/Part:BBa_K801093 BBa_K801093], [https://parts.igem.org/Part:BBa_K801094 BBa_K801094], [https://parts.igem.org/Part:BBa_K801095 BBa_K801095], [https://parts.igem.org/Part:BBa_K801096 BBa_K801096], [https://parts.igem.org/Part:BBa_K801097 BBa_K801097], [https://parts.igem.org/Part:BBa_K801098 BBa_K801098]
 '''Cloning details:'''<br>