Difference between revisions of "Part:BBa K741017"
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<partinfo>BBa_K741017 short</partinfo> | <partinfo>BBa_K741017 short</partinfo> | ||
The fusion protein crogfp is consistently being produced. If there is IPTG exists, IPTG will activates the promoter plac and the protein CI will hence activates the promoter pRM to express antisense RNA anticro. Thus the production of protein crogfp will be inhibited. | The fusion protein crogfp is consistently being produced. If there is IPTG exists, IPTG will activates the promoter plac and the protein CI will hence activates the promoter pRM to express antisense RNA anticro. Thus the production of protein crogfp will be inhibited. | ||
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| + | The fusion protein crogfp is consistently being produced. When the promoter plac is activated by IPTG, protein CI will be expressed to activate pRM. Then antisense RNA of cro will be expressed to inhibit the expression of CroGFP.This part is used to measure the function of pRM-anticro. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 07:58, 3 October 2012
plac-RBS-cI-T-pRM-anticro-T-pCon(0.244)-RBS-crogfp-T
The fusion protein crogfp is consistently being produced. If there is IPTG exists, IPTG will activates the promoter plac and the protein CI will hence activates the promoter pRM to express antisense RNA anticro. Thus the production of protein crogfp will be inhibited.
The fusion protein crogfp is consistently being produced. When the promoter plac is activated by IPTG, protein CI will be expressed to activate pRM. Then antisense RNA of cro will be expressed to inhibit the expression of CroGFP.This part is used to measure the function of pRM-anticro.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1589
Illegal NheI site found at 1612 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1370
Illegal BglII site found at 1706 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2500