Difference between revisions of "Part:BBa K902069:Design"

(→‎Design Notes)
(→‎References)
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===References===
 
===References===
 +
Egan, S. M., Schleif, R. F., & others. (1993). A regulatory cascade in the induction of rhaBAD. Journal of Molecular Biology, 234(1), 87-98.

Revision as of 07:47, 3 October 2012

RhaR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 559
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

RhaR is present at basal levels in cells. It binds with the inducer rhamnose to upregulate the rhaSR operon off the pRha rhamnose promoter. In turn, more RhaR and RhaS is produced, where RhaS can then activate the rhaBAD operon on the opposite side of the rhamnose promoter (Egan & Schleif, 1993).

Source

N/A

References

Egan, S. M., Schleif, R. F., & others. (1993). A regulatory cascade in the induction of rhaBAD. Journal of Molecular Biology, 234(1), 87-98.