Difference between revisions of "Part:BBa K902068:Design"
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===Source=== | ===Source=== | ||
| − | + | PCR amplified from Top Ten E. coli with Kapa polymerase. | |
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===References=== | ===References=== | ||
Egan, S. M., Schleif, R. F., & others. (1993). A regulatory cascade in the induction of rhaBAD. Journal of Molecular Biology, 234(1), 87-98. | Egan, S. M., Schleif, R. F., & others. (1993). A regulatory cascade in the induction of rhaBAD. Journal of Molecular Biology, 234(1), 87-98. | ||
Revision as of 07:42, 3 October 2012
RhaS
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 440
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 172
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This serves as regulatory element which activates expression of the pBAD operon on the pRha rhamnose promoter. In order for it to bind to pRha, the crp-cAMP complex must be bound to the promoter. Additionally, rhamnose will improve function of RhaS (Egan & Schlief, 1993).
In its native E. coli, the rhaS (on the rhaSR operon off the pRha promoter) is upregulated by the RhaR-rhamnose complex (Egan & Schlief, 1993).
Please view our [http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation#rhamnose Wiki] for more design details.
Source
PCR amplified from Top Ten E. coli with Kapa polymerase.
References
Egan, S. M., Schleif, R. F., & others. (1993). A regulatory cascade in the induction of rhaBAD. Journal of Molecular Biology, 234(1), 87-98.