Difference between revisions of "Part:BBa K847210"

Revision as of 07:39, 3 October 2012

polA (DNA Polymerase I) DnaA-activated promoter

The polA promoter (polAP) is a DNA-replication dependent promoter.

Usage and Biology

DnaA is the central initiator of DNA replication in E. coli and other prokaryotic organisms, but importantly, it also functions as a transcription factor that can suppress or activate transcription of genes by binding to the DnaA box, a 9bp consensus sequence (Messer 1997). Since DnaA expression is dependent on the growth conditions of the cell (Chiaramello 1990), genes regulated by DnaA are transitively growth dependent. One such DnaA-dependent gene is polA, which codes for DNA Polymerase I, active in DNA replication (Quiñones 1997).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 144
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K847210 short</partinfo>
-The nrd promoter (nrdP) is a cell-division dependent promoter.
+The polA promoter (polAP) is a DNA-replication dependent promoter.
 ===Usage and Biology===
-The nrd operon in E. coli expresses ribonucleotide reductase, an enzyme that reduces ribonucleotides into deoxyribonucleotides and is involved in bacterial cell cycle (Sun 1994). The promoter region is highly regulated and contains several DnaA boxes, indicating that activity is partly affected by initiation of DNA replication (Messer 2002). The promoter for this operon was found to begin activation during initiation of DNA replication and is cell-cycle dependent (Sun 1992).
+DnaA is the central initiator of DNA replication in E. coli and other prokaryotic organisms, but importantly, it also functions as a transcription factor that can suppress or activate transcription of genes by binding to the DnaA box, a 9bp consensus sequence (Messer 1997). Since DnaA expression is dependent on the growth conditions of the cell (Chiaramello 1990), genes regulated by DnaA are transitively growth dependent. One such DnaA-dependent gene is polA, which codes for DNA Polymerase I, active in DNA replication (Quiñones 1997).
 <span class='h3bb'>Sequence and Features</span>