Difference between revisions of "Part:BBa K847210"
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<partinfo>BBa_K847210 short</partinfo>
 
<partinfo>BBa_K847210 short</partinfo>
  
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The nrd promoter (nrdP) is a cell-division dependent promoter.
  
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===Usage and Biology===
 
===Usage and Biology===
  
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The nrd operon in E. coli expresses ribonucleotide reductase, an enzyme that reduces ribonucleotides into deoxyribonucleotides and is involved in bacterial cell cycle (Sun 1994). The promoter region is highly regulated and contains several DnaA boxes, indicating that activity is partly affected by initiation of DNA replication (Messer 2002). The promoter for this operon was found to begin activation during initiation of DNA replication and is cell-cycle dependent (Sun 1992).
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K847210 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K847210 SequenceAndFeatures</partinfo>

Revision as of 07:34, 3 October 2012

polA (DNA Polymerase I) DnaA-activated promoter

The nrd promoter (nrdP) is a cell-division dependent promoter.

Usage and Biology

The nrd operon in E. coli expresses ribonucleotide reductase, an enzyme that reduces ribonucleotides into deoxyribonucleotides and is involved in bacterial cell cycle (Sun 1994). The promoter region is highly regulated and contains several DnaA boxes, indicating that activity is partly affected by initiation of DNA replication (Messer 2002). The promoter for this operon was found to begin activation during initiation of DNA replication and is cell-cycle dependent (Sun 1992).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]