Difference between revisions of "Part:BBa K902035:Design"
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===References===
 
===References===
 +
 +
Kilbane, J.J. Microbial biocatalyst developments to upgrade fossil fuels. Current Opinion in Biotechnology, 17(3):305-314. 2006

Revision as of 06:28, 3 October 2012

AmdA Coding Sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 590
    Illegal XhoI site found at 82
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 90
    Illegal NgoMIV site found at 1295
    Illegal NgoMIV site found at 1401
    Illegal AgeI site found at 468
    Illegal AgeI site found at 652
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 326
    Illegal BsaI site found at 867


Design Notes

Nucleotide 72 was mutated from a G in the native organism to an A in our construct. This causes a silent mutation which does not affect the amino acid sequence.


Source

Gene was amplified using PCR from a Rhodococcus erythropolis strain 417 genomic prep template.

References

Kilbane, J.J. Microbial biocatalyst developments to upgrade fossil fuels. Current Opinion in Biotechnology, 17(3):305-314. 2006