Difference between revisions of "Part:BBa K778007"
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[[Image:UT Tokyo H2 1001+cm.png]] | [[Image:UT Tokyo H2 1001+cm.png]] | ||
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| + | (The modified fhlA(E363G) generator part is [[Part:BBa K778008]].) | ||
Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli/System] but the results in this wiki is older than this figure above. | Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli/System] but the results in this wiki is older than this figure above. | ||
Latest revision as of 05:39, 3 October 2012
FhlA-generator (pLac-RBS-fhlA-d.term)
pLac-RBS-fhlA-d.term
FhlA generator (pLac)
FhlA (Part:BBa K778001) is the transcriptional activator of hycAp (Part:BBa K778000). E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)).
This BioBrick part can be used to overexpress FhlA with IPTG induction for enhanced hydrogen production.
(The modified fhlA(E363G) generator part is Part:BBa K778008.)
Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli/System] but the results in this wiki is older than this figure above.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2178
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1457
- 1000COMPATIBLE WITH RFC[1000]
