Difference between revisions of "Part:BBa K902034"

Revision as of 05:32, 3 October 2012

CarAd Coding Sequence

The carAd gene in Pseudomonas putida codes for the ferredoxin reductase component of carbazole-1,9-dioxygenase. Together with carAa and carAc it forms the carbazole-1,9-dioxygenase (CARDO) enzyme which converts carbazole into 2'-aminobiphenyl-2,3-diol by cleaving one C-N bond in the original carbazole molecule.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 63
Illegal SapI.rc site found at 51

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-The <i>carAd</i> gene in <i>Pseudomonas putida</i> codes for the ferredoxin reductase component of carbazole-1,9-dioxygenase. Together with <i>carAa</i> and <i>carAc</i> it forms the carbazole-1,9-dioxygenase (CARDO) enzyme which converts carbazole into 2'-aminobiphenyl-2,3-diol by cleaving one C-N bond in the original carbazole molecule.
+The <i>carAd</i> gene in <i>Pseudomonas putida</i> codes for the ferredoxin reductase component of carbazole-1,9-dioxygenase. Together with <a href="https://parts.igem.org/Part:BBa_K902032"><i>carAa</i></a> and <a href="https://parts.igem.org/Part:BBa_K902033"><i>carAc</i></a> it forms the carbazole-1,9-dioxygenase (CARDO) enzyme which converts carbazole into 2'-aminobiphenyl-2,3-diol by cleaving one C-N bond in the original carbazole molecule.
 </html>[[Image:CarA pathway Calgary12.jpg|center]]