Difference between revisions of "Part:BBa K323066:Experience"

Revision as of 05:10, 3 October 2012

We used this part in combination with parts BBa_K323132 and BBa_K323135 and showed that this DNA program leads to higher yields of violacein production. Check results.

Applications of BBa_K323066

Experience by 2012 iGEM NCTU_FORMOSA

The design of our biobricks(Part:BBa_K887000) has the same order of zinc finger as 2010 Slovenia iGEM team, so we decided to use the DNA program (Part:BBa_K323066)

However, after sequencing, we found that there is a deletion of a base pair in the zif268 biding site.

So, we designed a complementary primer to insert the lost base pair. After PCR of point mutation, we sequence it again.

And the outcome is below

As a result, we successfully correct the lost G base at the zif268 binding site.

User Reviews

UNIQc86c29bcf60ce83b-partinfo-00000000-QINU UNIQc86c29bcf60ce83b-partinfo-00000001-QINU

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+As a result, we successfully correct the lost G base at the zif268 binding site.
 ===User Reviews===
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