Difference between revisions of "Part:BBa K778001"

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<!-- [[Image:UT Tokyo Fig diagram 640 480.png]]-->
 
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[[Image:UT Tokyo H2 1001.png]]
 
  
 
FhlA is the transcriptional activator of hycAp [[Part:BBa K778000]] .
 
FhlA is the transcriptional activator of hycAp [[Part:BBa K778000]] .
 
E.coli overexpressing FhlA produce H<sub>2</sub> (since hycAp is the promoter of FHL(formate hydrogen lyase)).
 
E.coli overexpressing FhlA produce H<sub>2</sub> (since hycAp is the promoter of FHL(formate hydrogen lyase)).
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[[Image:UT Tokyo H2 1001.png]]
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(The full construct in this experiment is [[Part:BBa K778007]] and
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The modified fhlA(E363G) part is [[Part:BBa K778002]].)
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Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli] but the results in this wiki is older than this figure above.  
 
Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli] but the results in this wiki is older than this figure above.  
  
The modified fhlA(E363G) part is [[Part:BBa K778002]].
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 04:54, 3 October 2012

fhlA: a transcriptional activator for enhanced hydrogen production


FhlA is the transcriptional activator of hycAp Part:BBa K778000 . E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)).

UT Tokyo H2 1001.png

(The full construct in this experiment is Part:BBa K778007 and The modified fhlA(E363G) part is Part:BBa K778002.)

Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli] but the results in this wiki is older than this figure above.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2096
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1375
  • 1000
    COMPATIBLE WITH RFC[1000]


Safety.png