Difference between revisions of "Part:BBa K778008"
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FhlA-E363G generator (pLac) | FhlA-E363G generator (pLac) | ||
| − | FhlA is the | + | FhlA ([[Part:BBa K778001]]) is the transcriptional activator of hycAp ([[Part:BBa K778000]]). |
| − | E.coli overexpressing FhlA produce | + | ''E.coli'' overexpressing FhlA produce H<sub>2</sub> (since hycAp is the promoter of FHL(formate hydrogen lyase)). |
fhlA-E363G has one point mutation (A to G, glutamic acid to glycine). | fhlA-E363G has one point mutation (A to G, glutamic acid to glycine). | ||
| − | This | + | This BioBrick part can be used to overexpress FhlA-E363G with IPTG induction for enhanced hydrogen production and for more H<sub>2</sub> production than [[Part:BBa K778001|FhlA(wild-type) generator]]. |
| − | + | [[Image:UT Tokyo H2 1001.png]] | |
| + | Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli/System] but the results in this wiki is older than this figure above. | ||
Revision as of 04:42, 3 October 2012
FhlA-E363G generator (pLac-RBS-fhlA363-d.term)
plac-rbs-fhlA363-d.term
FhlA-E363G generator (pLac)
FhlA (Part:BBa K778001) is the transcriptional activator of hycAp (Part:BBa K778000). E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)).
fhlA-E363G has one point mutation (A to G, glutamic acid to glycine).
This BioBrick part can be used to overexpress FhlA-E363G with IPTG induction for enhanced hydrogen production and for more H2 production than FhlA(wild-type) generator.
Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli/System] but the results in this wiki is older than this figure above.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2178
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1457
- 1000COMPATIBLE WITH RFC[1000]
