Difference between revisions of "Part:BBa K902048"

Latest revision as of 04:32, 3 October 2012

Constitutive XylE generator

XylE codes for the extra-diol dioxygenase called catechol 2,3-dioxygenase¹. This enzyme converts catechol to 2-hydroxymuconic semialdehyde, by cleaving catechol adjacent to the two hydroxyl groups¹.Because catechol is colourless and 2-HMS is a yellow compound visual assays can be used to monitor the activity of XylE. The tetR promoter (R0040) was used instead of the glucose repressible cstA promoter (K118011) so that the transformed cells could be grown in media containing glucose and XylE could still be expressed.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 399
Illegal NgoMIV site found at 571
Illegal AgeI site found at 922
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K902048 short</partinfo>
-XylE codes for the extra-diol dioxygenase called catechol 2,3-dioxygenase.  This enzyme converts catechol to 2-hydroxymuconic semialdehyde, by cleaving catechol adjacent to the two hydroxyl groups. Because catechol is colourless and 2-HMS is a yellow compound visual assays can be used to monitor the activity of XylE.  The tetR promoter (R0040) was used instead of the glucose repressible cstA promoter (K118011) so that the transformed cells could be grown in media containing glucose and XylE could still be expressed.
+XylE codes for the extra-diol dioxygenase called catechol 2,3-dioxygenase<sup>1</sup>.  This enzyme converts catechol to 2-hydroxymuconic semialdehyde, by cleaving catechol adjacent to the two hydroxyl groups<sup>1</sup>.Because catechol is colourless and 2-HMS is a yellow compound visual assays can be used to monitor the activity of XylE.  The tetR promoter (R0040) was used instead of the glucose repressible cstA promoter (K118011) so that the transformed cells could be grown in media containing glucose and XylE could still be expressed.
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