Difference between revisions of "Part:BBa K778007"
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FhlA generator (pLac) | FhlA generator (pLac) | ||
| − | FhlA is the | + | FhlA is the transcriptional activator of hycAp [[Part:BBa K778000]] . |
E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)). | E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)). | ||
| − | This | + | This BioBrick part can be used to overexpress FhlA with IPTG induction for enhanced hydrogen production. |
<!--[[Image:UT_Tokyo_Fig_diagram_640_480.png]]--> | <!--[[Image:UT_Tokyo_Fig_diagram_640_480.png]]--> | ||
[[Image:UT Tokyo H2 1001.png]] | [[Image:UT Tokyo H2 1001.png]] | ||
| − | Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli/System] | + | Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli/System] but the result in this wiki is old. |
Revision as of 04:30, 3 October 2012
FhlA-generator (pLac-RBS-fhlA-d.term)
pLac-RBS-fhlA-d.term
FhlA generator (pLac)
FhlA is the transcriptional activator of hycAp Part:BBa K778000 . E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)).
This BioBrick part can be used to overexpress FhlA with IPTG induction for enhanced hydrogen production.
Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli/System] but the result in this wiki is old.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2178
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1457
- 1000COMPATIBLE WITH RFC[1000]
