Difference between revisions of "Part:BBa K902032:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K902032 short</partinfo> | <partinfo>BBa_K902032 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
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| + | Source gene has a GTG start codon rather than an ATG start codon. Since the A in an ATG start codon is required to complete the biobrick XbaI site, this was mutated in our construct. | ||
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===Source=== | ===Source=== | ||
| − | + | Gene was amplified by PCR using <i>Pseudomonas putida</i> plasmid pCAR1.2 as a template | |
===References=== | ===References=== | ||
Revision as of 04:23, 3 October 2012
CarAa Coding Sequence
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Source gene has a GTG start codon rather than an ATG start codon. Since the A in an ATG start codon is required to complete the biobrick XbaI site, this was mutated in our construct.
Source
Gene was amplified by PCR using Pseudomonas putida plasmid pCAR1.2 as a template