Difference between revisions of "Part:BBa P1010:Experience"

Revision as of 20:07, 8 June 2006

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_P1010

This is the part that is present inside the BioBricks insertion site in many of the pSB series of plasmids. It is sufficient for positive selection even in pSB2* series plasmids in the absence of inducer (~4-5 copies per cell). During assembly work at the Registry, we have found that point mutations (particularly frame shifts) occur at a high enough rate that several colonies will survive during a normal transformation. -Randy

User Reviews

UNIQdf0ff683927745a6-partinfo-00000000-QINU

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Antiquity

This review comes from the old result system and indicates that this part worked in some test.

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 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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 ===Applications of BBa_P1010===
+This is the part that is present inside the BioBricks insertion site in many of the pSB series of plasmids.  It is sufficient for positive selection even in pSB2* series plasmids  in the absence of inducer (~4-5 copies per cell).
+During assembly work at the Registry, we have found that point mutations (particularly frame shifts) occur at a high enough rate that several colonies will survive during a normal transformation. -Randy
 ===User Reviews===