Difference between revisions of "Part:BBa K902034:Design"
Line 12: Line 12:
 
===Source===
 
===Source===
  
−
Pseudomonas putida plasmid pCAR1.2
+
Gene was amplified by PCR using <i>Pseudomonas putida</i> plasmid pCAR1.2 as a template
  
 
===References===
 
===References===

Revision as of 04:18, 3 October 2012

CarAd Coding Sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 63
    Illegal SapI.rc site found at 51


Design Notes

Site directed mutagenesis was required to remove a NotI site. Nucleotide 657 was changed from a G in the native DNA to an A in our construct. This was a conservative mutation which had no effect on the amino acid output.


Source

Gene was amplified by PCR using Pseudomonas putida plasmid pCAR1.2 as a template

References