Difference between revisions of "Part:BBa K935002"
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<partinfo>BBa_K935002 short</partinfo> | <partinfo>BBa_K935002 short</partinfo> | ||
| − | This part consists of a ArsR generator (BBa_K935001)under the control of pArs coupled to pArs and RFP as a reporter. This composite part was designed to quench the basal transcription of pArs and quantitatively raise the threshold of induction. | + | This part consists of a ArsR generator (BBa_K935001)under the control of pArs coupled to pArs and RFP as a reporter. This composite part was designed to quench the basal transcription of pArs and quantitatively raise the threshold of induction of RFP Expression. pArs-RFP was acquired from BBaK190015 and therefor there is no scar between the promoter and reporter allowing for an reporter substitution. |
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Revision as of 04:03, 3 October 2012
E. coli ArsR under the control of pArs coupled with pArs + RFP
This part consists of a ArsR generator (BBa_K935001)under the control of pArs coupled to pArs and RFP as a reporter. This composite part was designed to quench the basal transcription of pArs and quantitatively raise the threshold of induction of RFP Expression. pArs-RFP was acquired from BBaK190015 and therefor there is no scar between the promoter and reporter allowing for an reporter substitution.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 255
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1316
Illegal AgeI site found at 1428 - 1000COMPATIBLE WITH RFC[1000]