Difference between revisions of "Part:BBa K935001"

Latest revision as of 03:49, 3 October 2012

E. coli chromosomal ars promoter with arsR repressor gene and double terminator (B0010-B0012)

This part consists of the composite part BBa_J33201 designed by the 2006 Edinburgh Team which contains the promoter of the E. coli JM109 chromosomal arsenic detoxification operon (pArs), an RBS, and the gene for the ArsR repressor binding protein followed by an ORF. To create an ArsR generator under the control of the pArs we added a double terminator. This generator directs the production of ArsR at a basal transcription rate and this production will increase proportionally n the cell with an increase of a internal concentration of sodium arsenate or sodium arsenite (see also 2009 Gronigen for details: http://2009.igem.org/Team:Groningen/Modelling/Characterization)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 255
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 03:46, 3 October 2012 (view source) Geotwaddle (Talk \| contribs) ← Older edit		Latest revision as of 03:49, 3 October 2012 (view source) Geotwaddle (Talk \| contribs)
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	<partinfo>BBa_K935001 short</partinfo>		<partinfo>BBa_K935001 short</partinfo>

−	This part consists of the composite part BBa_J33201 designed by the 2006 Edinburgh Team which contains the promoter of the E. coli JM109 chromosomal arsenic detoxification operon (pArs), an RBS, and the gene for the ArsR repressor binding protein followed by an ORF. To create an ArsR generator under the control of the pArs we added a double terminator. This generator directs the production of ArsR at a basal transcription rate and this production will increase proportionally with an increase ~~in an~~ internal concentration of sodium arsenate or sodium arsenite (see also 2009 Gronigen for details: http://2009.igem.org/Team:Groningen/Modelling/Characterization)	+	This part consists of the composite part BBa_J33201 designed by the 2006 Edinburgh Team which contains the promoter of the E. coli JM109 chromosomal arsenic detoxification operon (pArs), an RBS, and the gene for the ArsR repressor binding protein followed by an ORF. To create an ArsR generator under the control of the pArs we added a double terminator. This generator directs the production of ArsR at a basal transcription rate and this production will increase proportionally n the cell with an increase of a internal concentration of sodium arsenate or sodium arsenite (see also 2009 Gronigen for details: http://2009.igem.org/Team:Groningen/Modelling/Characterization)