Difference between revisions of "Part:BBa K902035:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K902035 short</partinfo>
 
<partinfo>BBa_K902035 short</partinfo>
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===Design Notes===
 
===Design Notes===
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none
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Nucleotide 72 was mutated from a G in the native organism to an A in our construct. This causes a silent mutation which does not affect the amino acid sequence.
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===Source===
 
===Source===
  
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me
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Gene was amplified using PCR from a Rhodococcus erythropolis strain 417 genomic prep template.
  
 
===References===
 
===References===

Revision as of 03:41, 3 October 2012

AmdA Coding Sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 590
    Illegal XhoI site found at 82
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 90
    Illegal NgoMIV site found at 1295
    Illegal NgoMIV site found at 1401
    Illegal AgeI site found at 468
    Illegal AgeI site found at 652
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 326
    Illegal BsaI site found at 867


Design Notes

Nucleotide 72 was mutated from a G in the native organism to an A in our construct. This causes a silent mutation which does not affect the amino acid sequence.


Source

Gene was amplified using PCR from a Rhodococcus erythropolis strain 417 genomic prep template.

References