Difference between revisions of "Part:BBa J45006:Design"

(→‎Design Notes)
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===Source===
 
===Source===
−
 
+
Source species: ''S. cerevisiae''
 
Sequence obtained from Genbank, accession number [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=Nucleotide&dopt=GenBank&val=1420813 Z75285]
 
Sequence obtained from Genbank, accession number [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=Nucleotide&dopt=GenBank&val=1420813 Z75285]
  

Revision as of 19:54, 8 June 2006


alcohol acetyltransferase I (ATF1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 99
    Illegal BamHI site found at 1426
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 526
    Illegal SapI.rc site found at 1534


Design Notes

Eliminated EcoRI site at base pair 412 by changing base pair 414 from A to G

Source

Source species: S. cerevisiae Sequence obtained from Genbank, accession number [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=Nucleotide&dopt=GenBank&val=1420813 Z75285]

References

<biblio>

    • Horton03 pmid=12937998

</biblio>